pSHK4大肠杆菌与副猪嗜血杆菌穿梭载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pSHK4大肠杆菌与副猪嗜血杆菌穿梭载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
Shuttle vector pSHK4 for escherichia coli and haemophilus parasuis
The shuttle vector is named as pSHK4 and comprises a nucleotide sequence expressed by a sequence table SEQ ID NO:1, wherein a coding area of the pSHK4 is located at the sequence corresponding to 927-1742 bit sequence of the nucleotide sequence and 271 amino acids are coded. The shuttle vector provided by the invention has the advantages that a duplicated starting locus of natural plasmids in a bacillus separating strain of the haemophilus parasuis is utilized to construct the shuttle vector suitable for the haemophilus parasuis for the first time, the shuttle vector has the characteristics of stable hereditary in pathogenic bacteria and can be used for performing genetic operation on the haemophilus parasuis, and the shuttle vector is a basic tool for researching a toxic factor of the haemophilus parasuis and has a significance in revealing a pathogenic molecular pathopoiesia mechanism.
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