pcmv4-blam-vpr BioVector NTCC质粒载体菌种细胞基因保藏中心

pcmv4-blam-vpr BioVector NTCC质粒载体菌种细胞基因保藏中心
Vector backbone pCMV4
(Search Vector Database)
Backbone manufacturer Andersson,S. et al, J. Biol. Chem. 264 (14), 8222-8229 (1989)
Backbone size w/o insert (bp) 4874
Vector type Mammalian Expression
GROWTH IN BACTERIA
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number High Copy
GENE/INSERT
Gene/Insert name BlaM-Vpr
Species Fusion of BlaM and HIV-1 Vpr
Insert Size (bp) 1100
Entrez Gene vpr (a.k.a. HIV1gp4)
CLONING INFORMATION
Cloning method Restriction Enzyme
5′ cloning site HindIII (unknown if destroyed)
3′ cloning site HindIII (unknown if destroyed)
5′ sequencing primer CMV forward: cgcaaatgggcggtaggcgtg
3′ sequencing primer hGH-pA-R
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net