pBait-AbAi BioVector NTCC质粒载体菌种细胞基因保藏中心

pBait-AbAi BioVector NTCC质粒载体菌种细胞基因保藏中心
Yeast one-hybrid system
Create your bait reporter strain by homologous recombination into the genome of Y1HGold. The inactive ura3-
52 locus of Y1HGold consists of an irreversible transposon disruption that can only be repaired by homologous recombination
with the wild type URA3 gene, which is provided by the pBait-AbAi vector. Transformation of Y1HGold with a
pBait-AbAi vector linearized with BstBI or BbsI, results in colonies that can grow in the absence of uracil on SD/-Ura agar
plates. These colonies also contain a stable Bait-AbAi reporter that can be used to screen for protein-DNA interactions.
Your target-reporter construct (pBait-AbAi) should contain at least one copy of the DNA target element inserted
upstream of the AbAr reporter gene in pAbAi. Numerous studies have indicated
that the most effective constructs contain at least three tandem copies of the DNA target. Although three copies may
be preferred, published reports have demonstrated that a single copy may also suffice in certain cases. Tandem copies
may be generated by different methods, but we have found oligonucleotide synthesis to be the most convenient and
reliable, especially since well-defined regulatory elements are usually <20 bp.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net