pDR244 Cre Ts枯草芽孢杆菌无痕敲除（cre-lox敲除）载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥39862
- 货 号：pDR244 Cre Ts枯草芽孢杆菌无痕敲除（cre-lox敲除）载体质粒
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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pDR244 Cre Ts枯草芽孢杆菌无痕敲除（cre-lox敲除）载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
cre/lox-Mediated Loop-outProtocol for pDR244
(1)Transform pDR224 into one of the Bacillus subtilis BKE collection or any otherstrain harboring a
loxP-ankedantibiotic resistance cassette with selection for spectinomycin resistance at30°C
(2)Transfer several transformant colonies to plain LB plates and incubateovernight at 42°C
(3)Screen resulting colonies for plasmid curing (spectinomycin sensitivity) andthe antibiotic
resitancecassette (erythromycin sensitivity).
(4)Streak for fresh single colonies and then confirm loss of the cassette by PCR
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
cre/lox-Mediated Loop-outProtocol for pDR244
(1)Transform pDR224 into one of the Bacillus subtilis BKE collection or any otherstrain harboring a
loxP-ankedantibiotic resistance cassette with selection for spectinomycin resistance at30°C
(2)Transfer several transformant colonies to plain LB plates and incubateovernight at 42°C
(3)Screen resulting colonies for plasmid curing (spectinomycin sensitivity) andthe antibiotic
resitancecassette (erythromycin sensitivity).
(4)Streak for fresh single colonies and then confirm loss of the cassette by PCR
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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