pG-Sable 吉利蒙假丝酵母吉利蒙念珠菌穿梭表达载体Candida guilliermondii BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：pG-Sable 吉利蒙假丝酵母吉利蒙念珠菌穿梭表达载体Candida guilliermondii
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pG-Sable 吉利蒙假丝酵母吉利蒙念珠菌穿梭表达载体Candida guilliermondii BioVector NTCC质粒载体菌种细胞基因保藏中心
Selection and characterization of phleomycinresistant
colonies after direct transformation of Candida guilliermondii
with plasmid pG-Sable. Structure of pG-Sable (* indicates single-site
restriction enzymes) and selection of C. guilliermondii cells (ATCC
6260) on phleomycin-containing plates after transformation with
either sterile water (control) or 10 lg of linearized pG-Sable. Plates
were incubated for three days at 35 °C. The ApaI unique site was
used for linearization before transformation. The gel image shows the
results of yeast colony PCR on a subset of 16 randomly selected
clones performed using specific primers (BleS and BleR) to amplify the
Sable sequence.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
Selection and characterization of phleomycinresistant
colonies after direct transformation of Candida guilliermondii
with plasmid pG-Sable. Structure of pG-Sable (* indicates single-site
restriction enzymes) and selection of C. guilliermondii cells (ATCC
6260) on phleomycin-containing plates after transformation with
either sterile water (control) or 10 lg of linearized pG-Sable. Plates
were incubated for three days at 35 °C. The ApaI unique site was
used for linearization before transformation. The gel image shows the
results of yeast colony PCR on a subset of 16 randomly selected
clones performed using specific primers (BleS and BleR) to amplify the
Sable sequence.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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