pNitET-SacB-kan分枝杆菌基因重组辅助质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pNitET-SacB-kan分枝杆菌基因重组辅助质粒载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
pNitET-SacB-kan分枝杆菌基因重组辅助质粒载体，表达分枝杆菌噬菌体Che9c RecET，带有Kan抗性。在耻垢分支杆菌和结核分枝杆菌中分别经caprolactam和IVN诱导表达。
Expression of the phage Che9c RecET system for Recombineering in mycobacteria
This plasmid can be used to promote recombineering in M. smegmatis or M. tuberculosis. The ReET gene are induced with caprolactam in M. smegmatis and with isovaleronitrile (IVN) for M. tuberculosis.

For original description of this methodology, see:
van Kessel, J.C. & Hatfull, G.F. Recombineering in Mycobacterium tuberculosis. Nat Methods 4, 147-152 (2007).
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