pOpt_Clover_Paro衣藻荧光表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pOpt_Clover_Paro衣藻荧光表达载体质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
pOpt_Clover_Paro衣藻荧光表达载体质粒，带有Clover绿色荧光报告基因，HSP70A-RBCS2-lnl启动子，RBCS2内含子2，衣藻筛选标记为paromomycin aphVIII
Vector: pBluescript backbone, pOptimized synthetic Chlamydomonas expression cassettes

Origin: synthetic construct (DNA synthesis and cloning)

Insert pOptimized template vector containing the Clover (a GFP variant) reporter expression cassette and an antibiotic selection marker expression cassette. Both cassettes contain the HSP70A-RBCS2-InI promoter and RBCS2 3’UTR surrounding either the reporter or the marker. Each reporter contains intron 2 of RBCS2 within its nuclear sequence. Selection marker for Chlamydomonas is paromomycin resistance aphVIII (Sizova et al. 2001).

Host strain: DH5α

Selection: Ampicillin resistance for E. coli culture

Comment: Please follow naming convention for derivative vectors using underscores between new added elements.
Individual annotated vector maps are available in ApE format.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net