SSN-1 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

SSN-1 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell Line Name: SSN-1
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: SSN-1
Keywords: Fish striped snakehead fry, spontaneous production of c-type retrovirus
Cell Line Description: The fish cell line SSN-1 was initiated from whole fry tissue, Channa (Ophicephalus) striatus, commonly named 'striped snakehead'. SSN-1 spontaneously produce and release endogenous snakehead fish Mn²+- dependent C-type retrovirus. The cell line is suitable for fish virus isolation and is susceptible to epizootic ulcerative syndrome rhabdoviruses and piscine nodaviruses. SSN-1 was previously infected with mycoplasma but has been eradicated and tested negative after a minimum of 10 passages.
Species: Fish
Tissue of Origin: unknown
CellType: Fibroblast
Growth Mode: Adherent
Karyotype: Aneuploid
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10, 000 cells/cm² using 0.25% trypsin or trypsin/EDTA. Use trypsin very cautiously; 25-30°C, cells can be light sensitive therefore avoid prolonged exposure to bright light. When resuscitating a frozen ampoule of cells we recommend the cells are seeded at the higher seeding level i.e. 4 x10, 000/cm². Cells may take up to 10 days to reach 70% following resuscitation and subculture. Therefore media change flasks after 4-5 days to encourage growth.
Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping



Culture Medium: L15 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).
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