pcDNA-GADPH-Flag BioVector NTCC质粒载体菌种细胞基因保藏中心

pcDNA-GADPH-Flag BioVector NTCC质粒载体菌种细胞基因保藏中心
GAPDH cDNA ORF Clone, Human, C- Flag tag
种属
Human
NCBI 参考序列号
AF261085.1
参考序列ORF长度
1008 bp
序列描述
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 381 T>C and 885 T>C not causing the amino acid variation.
描述
Full length Clone DNA of Human glyceraldehyde-3-phosphate dehydrogenase with C terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
pcDNA-FLAG-Hyg
限制性酶切位点
KpnI + NotI (6kb + 1.06kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
抗生素(大肠杆菌)
Kanamycin
抗生素(哺乳动物细胞)
Hygromycin
应用
Stable or Transient mammalian expression
GAPDH Background Information
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.
Full Name
glyceraldehyde-3-phosphate dehydrogenase

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