Enterobacteria phage MS2噬菌体 BioVector NTCC质粒载体菌种细胞基因保藏中心
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- 货 号：Enterobacteria phage MS2噬菌体
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Enterobacteria phage MS2噬菌体 BioVector NTCC质粒载体菌种细胞基因保藏中心
Species:
Enterobacteria phage MS2
Taxonomy:
Order: Unassigned
Family: Leviviridae
Type species of the genus: Levivirus
Isolate:
MS2
NTCC No.:
PG13767
Strain designation:
PC-V3463
History:
<- Phabagen
Genbank accession numbers:
complete genome: NC_001417
Genomics:
Linear ssRNA. Phage has a very small genome (3.569 nucleotides); small stem-loops from the MS2 genome are very important for detection of RNA in living cells ("MS2 tagging": interaction of MS2 coat protein with stem-loop structure from the phage genome; used for biochemical purification of RNA-protein complexes)
Morphology:
Nonenveloped, isometric, icosahedral, small, tail-less phage, size approx. 25 nm
Life cycle:
virulent phage, lytic cycle
Plaques description:
large turbid plaques
Image:
Application:
Used in Expt. 49 in "Sourcebook of Experiments for the Teaching of Microbiology", eds. S.B. Primrose and A.C. Wardlaw, Academic Press (1982)
Risk group:
1
Phage propagation
Host bacterium:
Escherichia coli (Migula 1895) Castellani and Chalmers 1919
Cultivation conditions:
NZCYM-MEDIUM plus 2.0 mg of streptomycin per l, 37°C
NZCYM-MEDIUM
Casein hydrolysate 10.0 g
NaCl 5.0 g
Casamino acids (Difco) 1.0 g
Yeast extract 5.0 g
MgSO4 x 7 H2O 2.0 g
Maltose 2.0 g
Distilled water 1000.0 ml
Adjust pH to 7.5.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
Species:
Enterobacteria phage MS2
Taxonomy:
Order: Unassigned
Family: Leviviridae
Type species of the genus: Levivirus
Isolate:
MS2
NTCC No.:
PG13767
Strain designation:
PC-V3463
History:
<- Phabagen
Genbank accession numbers:
complete genome: NC_001417
Genomics:
Linear ssRNA. Phage has a very small genome (3.569 nucleotides); small stem-loops from the MS2 genome are very important for detection of RNA in living cells ("MS2 tagging": interaction of MS2 coat protein with stem-loop structure from the phage genome; used for biochemical purification of RNA-protein complexes)
Morphology:
Nonenveloped, isometric, icosahedral, small, tail-less phage, size approx. 25 nm
Life cycle:
virulent phage, lytic cycle
Plaques description:
large turbid plaques
Image:
Application:
Used in Expt. 49 in "Sourcebook of Experiments for the Teaching of Microbiology", eds. S.B. Primrose and A.C. Wardlaw, Academic Press (1982)
Risk group:
1
Phage propagation
Host bacterium:
Escherichia coli (Migula 1895) Castellani and Chalmers 1919
Cultivation conditions:
NZCYM-MEDIUM plus 2.0 mg of streptomycin per l, 37°C
NZCYM-MEDIUM
Casein hydrolysate 10.0 g
NaCl 5.0 g
Casamino acids (Difco) 1.0 g
Yeast extract 5.0 g
MgSO4 x 7 H2O 2.0 g
Maltose 2.0 g
Distilled water 1000.0 ml
Adjust pH to 7.5.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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