pECFP-YFP双荧光表达载体黄色青色荧光表达质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心

pECFP-YFP双荧光表达载体黄色青色荧光表达质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
pECFP-YFP双荧光表达载体黄色青色荧光表达质粒，带有ECFP和YFP荧光报告基因，Kan抗性，CMV启动子，G418细胞筛选标记。
Cloned DNA: Aequorea victoria green fluorescent protein DNA (GFP); yellow-green fluorescent variant (YFP) with modified 3' end
Aequorea victoria green fluorescent protein DNA (GFP); enhanced cyan fluorescent variant (ECFP), N-terminal
Xpress epitope; N-terminal
Enterokinase cleavage site (EK); N-terminal
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Escherichia coli β-lactamase promoter (amp)
Simian virus 40 early promoter (SV40 early)
Ribosome
binding site: Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp)
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA)
Selection marker: Neomycin (neo; G418; kanamycin (kan))
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pECFP-C1
Further information: The plasmid was constructed by cloning the YFP coding sequence into the BglII/HindIII opened pECFP-C1 vector.
The YFP sequence has a modified 3' end.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726795.1.
The YFP insert has been sequenced
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 37°C
This culture grows very slow: take 24 hours into account for saturated growth!
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net