pCC1BAC Vector BAC克隆质粒BAC文库构建载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pCC1BAC Vector BAC克隆质粒BAC文库构建载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
pCC1BAC Vector is
based on an innovative technology originally
developed in the laboratory of Dr. Waclaw
Szybalski1 and optimized at EPICENTRE.2
The
vector has two origins of replication – a singlecopy
E. coli F-factor replicon and a high-copy
origin of replication called “oriV”. Initially, replication
of CopyControl clones can be controlled by
the F-factor replicon so the vector is present at
one copy per cell. Maintaining clones at single
copy ensures insert stability and allows cloning
of toxic gene products (Figure 1, page 2).
Initiation of replication from oriV requires the trfA
gene product. CopyControl Vectors use a specifically
engineered E. coli host strain, TransforMax™
EPI300™ (available separately), which
contains a mutant trfA gene under tight control
of an inducible promoter. Addition of the CopyControl
Induction Solution to the growth medium
induces expression of trfA and subsequent amplification
of the clone to high-copy number. Induction
of CopyControl BAC clones from singlecopy
up to 10-20 copies per cell greatly improves
the yield and purity of BAC DNA for sequencing,
fingerprinting and other applications.
The CopyControl pCC1BAC Vector is derived
from pBeloBAC113 and EPICENTRE’s pIndigoBAC-5.
The vector has been linearized at a
unique restriction enzyme recognition site
(BamH I, Hind III or EcoR I), dephosphorylated,
and highly purified to ensure very low background.
Features of the vector include:
• Chloramphenicol-resistance as an antibiotic
selectable marker.
• E. coli F factor-based partitioning and singlecopy
number regulation system.
• oriV high-copy origin of replication.
• Primer binding sites for BAC-end sequencing
• Not I sites surrounding the BamH I, Hind III
and EcoR I cloning sites.
• Bacteriophage P1 loxP site for Cre-recombinase
cleavage.
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net