OPM-2 cell line人多发性骨髓瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

OPM-2 cell line人多发性骨髓瘤细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line: OPM-2 人多发性骨髓瘤细胞株 NTCC no.: NTCC-ACC50 Species: human (Homo sapiens) Cell type: multiple myeloma Origin: established from the peripheral blood of a 56-year-old woman with multiple myeloma (IgG lambda) in leukemic phase (relapse, terminal) in 1982; described to carry t(4;14) leading to IGH@-FGFR3 (IGH-FGFR3; IGH-MMSET) fusion gene Biosafety level: 1 NTCC Cell Culture Data: Morphology: single, round to polygonal cells in suspension Medium: 90% RPMI 1640 + 10% h.i. FBS Subculture: maintain at 0.3-0.7 x 106 cells/ml; split 1:2 to 1:8 every 2 days; keep at 20% FBS for the first week; seed out at ca. 1 x 106 cells/ml in a 6-well-plate or 24-well-plate Incubation: at 37 °C with 5% CO2 Doubling time: ca. 50-60 hours Harvest: maximal density at 1.0 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO NTCC Scientific Data: Mycoplasma: negative in DAPI, microbiological culture, PCR assays Immunology: CD3 -, CD10 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -; image Fingerprint: multiplex PCR of minisatellite markers revealed a unique DNA profile Species: confirmed as human with IEF of AST, MDH, NP Cytogenetics: human hypertriploid/hypotetraploid karyotype - 78(77-82)<3n>XX-X, +1, +1, +1, +2, +4, +5, -7, +9, +11, -14, +15, +16, +18, +19, -21, +2-3mar, add(1)(p11), add(1)(q11)x2, del(1)(p11)x2, del(1)(q32), t(4;14)(p16;q32.3), der(4)t(4;14)(p16;q32.3), add(5)(q11), add(5)(q15-21), add(5)(q35), der(8)t(1;8)(?q32;q24)x1-2, add(14)(q32.1), der(22)t(1;22)(q11;p13)x2 - sideline with del(7)(p15), del(10)(q22) - resembles published karyotype - carries cryptic t(4;14) associated with fusion of IGH@ (IGH) with FGFR3 (MMSET) Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV -, HTLV-I/II -, SMRV -
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