pSNAP-tag (T7)-2 BioVector NTCC质粒载体菌种细胞基因保藏中心

pSNAP-tag (T7)-2 BioVector NTCC质粒载体菌种细胞基因保藏中心
pSNAP-tag® (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag protein. Expression is under control of the IPTG inducible T7 promoter.

This plasmid is intended as an expression vector for SNAP-tag gene fusions in appropriate E. coli strains
The E. coli strain used for expression needs to provide T7 polymerase in order to achieve expression from the T7 promoter upstream of the gene
The vector can be used for expression of the SNAP-tag protein alone (20 kDa)
Gene of interest can be fused to the N-terminus or C-terminus of SNAP-tag
pSNAP-tag (T7)-2 Vector is an Escherichia coli expression plasmid encoding the SNAP-tag protein. Expression is under control of the IPTG inducible T7 promoter. The pSNAP-tag (T7)-2 Vector can also be used as a control plasmid expressing the SNAP-tag protein (20 kDa). The target gene should be cloned as a fusion to the N- or C-terminus of the SNAP-tag.

The SNAP-tag is a novel tool for protein research, allowing the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is a protein based on human O6-alkylguanine-DNA-alkyltransferase (hAGT)(1,2,3). SNAP-tag substrates are derivatives of benzyl purines and benzyl chloropyrimidines. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

There are two steps to using this system: sub cloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Cloning and expression of SNAP-tag fusion proteins are described in this document. The labeling of the fusion proteins with SNAP-tag substrates is described in the instructions supplied with SNAP-tag substrates.
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