BRIN-BD11 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥49862
- 货 号：BRIN-BD11 cell line细胞株
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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BRIN-BD11 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Keywords: A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line
Cell Line Description: BRIN-BD11 is a hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BD11 has been shown to be tumourigenic when transplanted into a SCID mouse host. BRIN-BD11 has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma. The cell line has applications in the study of pancreatic beta cell function.
Species: Rat
Tissue of Origin: Pancreatic islets
CellType: Epitheloid
Karyotype: Modal chromosome number 58 over 50 passages
Subculture Routine: Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2-4 x 104 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 20hrs. At confluence 105 cells/cm² can be expected.
Culture Medium: RPMI-1640 + 2mM Glutamine + 10% FCS
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
Keywords: A rat pancreatic Beta cell: RINm5F hybrid cell line. Insulin-secreting cell line
Cell Line Description: BRIN-BD11 is a hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BD11 has been shown to be tumourigenic when transplanted into a SCID mouse host. BRIN-BD11 has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma. The cell line has applications in the study of pancreatic beta cell function.
Species: Rat
Tissue of Origin: Pancreatic islets
CellType: Epitheloid
Karyotype: Modal chromosome number 58 over 50 passages
Subculture Routine: Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2-4 x 104 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 20hrs. At confluence 105 cells/cm² can be expected.
Culture Medium: RPMI-1640 + 2mM Glutamine + 10% FCS
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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