猪肺炎支原体济南强毒株Mycoplasma hyopneumoniae BioVector NTCC质粒载体菌种细胞基因保藏中心

猪肺炎支原体济南强毒株Mycoplasma hyopneumoniae BioVector NTCC质粒载体菌种细胞基因保藏中心
名称 猪肺炎支原体济南强毒株
种属 Mycoplasma hyopneumoniae
分离基物 喘气病猪
提供形式 冻干物
致病类型 猪流行性肺炎
安全等级 2
模式菌株 no
应用领域 检验疫苗
培养方法
培养基 Revised Mycoplasma Medium
生长条件 37
存储条件 真空冷冻干燥法;
详细说明
描述 山东农科院，济南株。喘气病猪，山东省济南市。强毒株，对猪的毒力为100MID/5mL,使猪致病（潜伏期为720天）；该株在猪肺气管内繁殖，使肺部产生融合性肺炎、电镜观察肺支气管纤毛严重损伤。检验疫苗。培养基21，37℃。
寄主名称 猪
致病对象 动物
传播途径 呼吸道
采集地 中国山东省济南市
Medium
Medium: Revised Mycoplasma Medium
Heart Infusion Broth (BD 238400).......................................7.5 g
Agar, Noble (BD 214230) (if necessary).............................10.0 g
DI Water…….......................................................................660.0 ml
Adjust for final pH 7.4 and autoclave at 121ºC.
Add aseptically the following filter-sterilized components:
10X Hanks' Balanced Salt Solution w/o
NaHCO3 or phenol red (see below) ....................................40.0 ml
0.25% Phenol red................................................................10.0 ml
Porcine serum (heat-inactivated).........................................200.0 ml
5 % Lactalbumin Hydrolysate, TC (BD 259962)
in 1 X Dulbecco's PBS (GIBCO 14040)...............................100.0 ml
Yeast Extract Solution (GIBCO 18180)................................20.0 ml
Hanks' Balanced Salt Solution (10X)
CaCl2 ..........................................1.4 g
KCl...............................................4.0 g
KH2PO4 ......................................0.6 g
MgCl2 x 6H2O .............................1.0 g
MgSO4 x 7H2O ...........................1.0 g
NaCl..............................................80.0 g
Na2HPO4 x 7H2O........................0.9 g
Glucose........................................10.0 g
DI Water to...................................1000.0 ml

Growth Conditions
Growth Conditions Temperature: 37°C
Atmosphere: Broth: Aerobic; Plates: 5% CO2

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