pSGFPS1金黄色葡萄球菌荧光表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pSGFPS1金黄色葡萄球菌荧光表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

A glycerol stock of Staphylococcus aureus (S. aureus), strain RN4220 containing the green fluorescent protein (GFP) reporter plasmid pSGFPS1, a derivative of the Escherichia coli (E. coli) - staphylococcal shuttle vector pKK30.

Plasmid pKK30 is a member of a set of shuttle vectors that retain stability over a number of generations in vitro and in vivo in Staphylococcus species without antibiotic selection, making them a powerful genetic tool. pKK30 contains the PsarAP1::dfrA trimethoprim resistance cassette, a blaZ transcriptional terminator (blaZTT), a plasmid single stranded origin (sso) required for plasmid replication, a double stranded origin (dso) required for plasmid maintenance in the absence of antibiotic selection, a repF gene encoding for the S. aureus Rep protein and the E. coli R6Kγ replication origin. pKK30 lacks the four predicted USA300-specific open reading frames allowing for use of pKK30 in non-USA300 isolates.

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