HCC-78人肺腺癌细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

HCC-78人肺腺癌细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line:
HCC-78
Species:
human (Homo sapiens)
Cell type:
non-small cell lung carcinoma
Origin:
established from the pleural effusion of a 65-year-old man with adenocarcinoma of the lung, typed as non-small cell lung carcinoma; cells are described in the literature to carry a ROS1 rearrangement; matched EBV+ B-lymphoblastoid cell line (B-LCL) is available (HCC-78BL)
Biosafety level:
1

Cell Culture Data:
Morphology:
large epitheloid cells growing in monolayers and aggregates; after trypsination cells are not always dispersed as single cells;
Image


Medium:
90% RPMI 1640 + 10% h.i. FBS
Subculture:
seed out 1-2 x 106 cells in 175 cm2; split confluent culture 1:2 twice a week using trypsin/EDTA
Incubation:
at 37 °C with 5% CO2
Doubling time:
ca. 60-70 hours
Harvest:
cell harvest of ca. 8-10 x 106 cells/175 cm2
Storage:
frozen with 70% medium, 20% FBS, 10% DMSO

Scientific Data:
Mycoplasma:
negative in microbiological culture, PCR assays
Immunology:
cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 (+), cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin +
Fingerprint:
fluorescent nonaplex PCR of short tandem repeat markers revealed a unique DNA profile
Species:
confirmed as human by PCR speciation and cytogenetics
Cytogenetics:
human flat-moded complex hypotetraploid karyotype with 6% polyploidy - 80-91<4n>XYY/XXYY, add(X)(q25)x2, add(1)(?p11), add(4)(p15), add(4)(q31), add(16)(q23), der(16)t(1;16)(p12;q23)x2, dup(16)(q11q13)add(16)(q13), add(19)(q13)x2, inc.
Viruses:
PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV

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