MIRJT7i-mND2-0 BioVector NTCC质粒载体菌种细胞基因保藏中心

MIRJT7i-mND2-0 BioVector NTCC质粒载体菌种细胞基因保藏中心
Product Name MIRJT7i-mND2-0
Alias mND2-0
Lot Number WB0248
Parent Material MIRJT7i-mND2-0-WB0119

Thaw Recommendation Thaw 1 vial into 1 well of a 6 well plate. WiCell recommends thawing using ROCK Inhibitor for best results.
Culture Platform Feeder Independent
Medium: E8 – passage using ROCK Inhibitor for best results.
Matrix: Matrigel
Protocol Feeder Independent E8 Medium Protocol
Passage Number p28
These cells were cultured for 27 passages prior to freeze, at least 7 passages in E8/Matrigel. WiCell adds +1 to
the passage number at freeze so that the number on the vial best represents the overall passage number of the
cells at thaw.
Intro：
We re-examine the individual components for human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) culture and formulate a cell culture system in which all protein reagents for liquid media, attachment surfaces and splitting are chemically defined. A major improvement is the lack of a serum albumin component, as variations in either animal- or human-sourced albumin batches have previously plagued human ESC and iPSC culture with inconsistencies. Using this new medium (E8) and vitronectin-coated surfaces, we demonstrate improved derivation efficiencies of vector-free human iPSCs with an episomal approach. This simplified E8 medium should facilitate both the research use and clinical applications of human ESCs and iPSCs and their derivatives, and should be applicable to other reprogramming methods.

【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net