SCLC-21H cell line细胞株ACC 372 BioVector NTCC质粒载体菌种细胞基因保藏中心
- 价 格：¥39862
- 货 号：SCLC-21H cell line细胞株ACC 372
- 产 地：北京
- BioVector NTCC典型培养物保藏中心
- 联系人：Dr.Xu, Biovector NTCC Inc.
电话：400-800-2947 工作微信:1843439339 (QQ同号)
邮件：Biovector@163.com
手机：18901268599
地址：北京
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SCLC-21H cell line细胞株ACC 372 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell line: SCLC-21H DSMZ no.: ACC 372Species: human (Homo sapiens)Cell type: small cell lung carcinoma Origin: established from the pleural effusion of a 46-year-old Caucasian man with small cell lung carcinoma; sister cell line of cell line SCLC-22H (DSM ACC 373) Reference(s): 14381, 14382Biosafety level: 1 Permissions and restrictions: A DSMZ Cell Culture Data: Morphology:growing in suspension in small or very big clusters (sheets) - cells are not adherent; imageMedium: 90% Dulbecco's MEM (4.5 g/L glucose) + 10% h.i. FBS Subculture: seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3 - 1.0 x 106 cells/ml; split 1:2 every 2-3 days; cells grow in clumps and are difficult to disperse; viability may decrease when dispersed too vigorously; cell counting may be difficult Incubation: at 37 °C with 5% CO2 Doubling time: ca. 2 days Harvest: cell harvest of ca. 1 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO DSMZ Scientific Data: Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays Immunology: cytokeratin (+), cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin (+) Fingerprint: identical DNA profiles of SCLC-16HC, SCLC-21H, SCLC-22H and SCLC-24H using multiplex PCR of minisatellite markers Species: confirmed as human with IEF of AST, LDH, NP Cytogenetics: human hypodiploid karyotype with 12% polyploidy - 42(40-42)<2n>XY, +1, -2, -5, +6, -10, +11, -17, -18, -19, -21, +2mar, del(1)(p22p32), add(5)(p15), del(6)(q14), add(8)(p21), add(11)(q24), del(11)(p11), t(12;17)(q10;q10), del(13)(q12q14), add(15)(q25) - resembles published karyotype (and that of SCLC-16HC) Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV -
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
Cell line: SCLC-21H DSMZ no.: ACC 372Species: human (Homo sapiens)Cell type: small cell lung carcinoma Origin: established from the pleural effusion of a 46-year-old Caucasian man with small cell lung carcinoma; sister cell line of cell line SCLC-22H (DSM ACC 373) Reference(s): 14381, 14382Biosafety level: 1 Permissions and restrictions: A DSMZ Cell Culture Data: Morphology:growing in suspension in small or very big clusters (sheets) - cells are not adherent; imageMedium: 90% Dulbecco's MEM (4.5 g/L glucose) + 10% h.i. FBS Subculture: seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3 - 1.0 x 106 cells/ml; split 1:2 every 2-3 days; cells grow in clumps and are difficult to disperse; viability may decrease when dispersed too vigorously; cell counting may be difficult Incubation: at 37 °C with 5% CO2 Doubling time: ca. 2 days Harvest: cell harvest of ca. 1 x 106 cells/ml Storage: frozen with 70% medium, 20% FBS, 10% DMSO DSMZ Scientific Data: Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays Immunology: cytokeratin (+), cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin (+) Fingerprint: identical DNA profiles of SCLC-16HC, SCLC-21H, SCLC-22H and SCLC-24H using multiplex PCR of minisatellite markers Species: confirmed as human with IEF of AST, LDH, NP Cytogenetics: human hypodiploid karyotype with 12% polyploidy - 42(40-42)<2n>XY, +1, -2, -5, +6, -10, +11, -17, -18, -19, -21, +2mar, del(1)(p22p32), add(5)(p15), del(6)(q14), add(8)(p21), add(11)(q24), del(11)(p11), t(12;17)(q10;q10), del(13)(q12q14), add(15)(q25) - resembles published karyotype (and that of SCLC-16HC) Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV -
【Supplier来源】BioVector NTCC Inc.
TEL：+86-010-53513060
【Website网址】 http://www.biovector.net
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