pTol2-CD41-GFP BioVector NTCC质粒载体菌种细胞基因保藏中心

pTol2-CD41-GFP BioVector NTCC质粒载体菌种细胞基因保藏中心
cd41 is a surface marker of early platelet progenitor and
mature platelet. Its expression has been noticed on the
potential HSCs in zebrafish, as indicated by the cd41:GFP
transgenic fish. The non-mobile pool of GFP+ cells between
the DA and PCV decreased when treated with scl-MO, but
not with cmpl-MO knockdown [34], implying that cd41:GFP
transgenic line may be a useful tool to study the origin and
transition routes of HSCs. Using this transgenic line,
Herbomel’s laboratory reported the living imaging of putative
HSCs, and found that without intra-aorta cell clusters in
AGM, the cd41:GFPlow HSCs emerge in the sub-aorta of the
mesenchyme, and enter the circulation through the axial vein
[10,32]. cd41:GFP+ cells are first detected by 33 to 35 hpf
between the dorsal aorta and the posterior cardinal vein, and
migrate to the CHT by 48 to 56 hpf. Around 54 to 56 hpf, the
GFP+ cells were found to colonize the nascent thymus
through the mesenchyme, not by circulation [32]. A novel
migration route was further identified from the cd41:GFP+
cells in AGM to the developing kidney by the pronephric
tubules at 48 to 96 hpf
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