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pEYFP-Mem细胞膜定位黄色荧光表达载体
启动子: | CMV |
复制子: | pUC ori,f1 ori |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞,荧光蛋白报告载体 |
质粒大小: | 4793bp |
原核抗性: | Kan |
筛选标记: | Neo |
克隆菌株: | DH5α |
培养条件: | 37℃,有氧 LB |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导,瞬时表达 |
5'测序引物: | 根据序列设计引物 |
3'测序引物: | 根据序列设计引物 |
pEYFP-Mem encodes a fusion protein consisting of the N-terminal 20 aminoacids of neuromodulin,also called GAP-43, and a yellow-green fluorescentvariant of the enhanced green fluorescent protein (EGFP). The neuromodulinfragment contains a signal for posttranslational palmitoylation of cysteines 3and 4 that targets EYFP to membranes. The EYFP gene contains four amino acidsubstitutions previously published as GFP-10C. The fluorescence excitationmaximum of EYFP is 513 nm, and the emission maximum is 527 nm (in theyellow-green region).
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