pBAD/His-B-iRFP BioVector NTCC质粒载体菌种细胞基因保藏中心

pBAD/His-B-iRFP BioVector NTCC质粒载体菌种细胞基因保藏中心
Biovector031855 pBAD/His-B-iRFP A phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging For additional protocols on using iRFP in other tissue types please review: Verkhusha. Deep-tissue photoacoustic tomography of a genetically encoded near-infrared fluorescent probe. Angewandte Chemie Int. Ed. 2012, 51: 1448-1451. • Vector backbone pBAD/His-B • Backbone manufacturer Invitrogen • Backbone size w/o insert (bp)4100 • Vector type Bacterial Expression • Bacterial Resistance(s) Ampicillin • Growth Temperature 37°C • Growth Strain(s) DH5alpha • Copy number Low Copy • Gene/Insert name iRFP • Alt name iRFP713 • Species Rhodopseudomonas palustris • Insert Size (bp) 948 • Mutation S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T and N290Y relative to RpBphP2 • GenBank ID JN247409 • PromoteraraBAD • Cloning methodRestriction Enzyme • 5′ cloning siteBglII (not destroyed) • 3′ cloning siteHindIII (not destroyed) • 5′ sequencing primerpBAD_fwd_primer
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