pLCA.66/2272抗性片段质粒

pLCA.66/2272抗性片段质粒
pLCA.66/2272基因打靶组装质粒BAC重组载体，带有pUDTK鼠源启动子+puro-delta TK融合基因，neomycin和Amp抗性基因。lox66 and lox2272位点用于Cre重组酶识别。
启动子: PGK promoter 复制子: ori，F1 ori 终止子: bGH poly(A) signal 质粒分类: 基因文库质粒；其它基因质粒；其它种源质粒 质粒大小: 6678bp 原核抗性: 氨苄青霉素Amp（100μg/ml） 筛选标记: 嘌呤霉素Puro 克隆菌株: DH5α等大肠杆菌 培养条件: 37℃，有氧，LB 质粒简介 This vector was designed for assembling gene targeting constructs using the BAC recombineering approach. The vector contains a puromycin-(delta)thymidine kinase fusion gene driven by the mouse phosphoglycerol kinase promoter (pUDTK) and a neomycin resistant gene driven by the bacterial EM7 promoter (EM7neo). These selectable markers are flanked by lox66 and lox2272 sites (Cre-recombinase recognition sequences) in a pBluescript KS+ backbone with a modified multi-cloning region.

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