pRL-TK哺乳海肾荧光素报告质粒

pRL-TK哺乳海肾荧光素报告质粒
启动子: HSV TK，T7 复制子: pUC ori 终止子: SV40 poly(A) signal 质粒分类: 哺乳细胞，信号通路报告载体 质粒大小: 4045bp 原核抗性: Amp 克隆菌株: DH5α 培养条件: 37℃，有氧 LB 表达宿主: 哺乳细胞 诱导方式: 无须诱导，瞬时表达 5'测序引物: T7:TAATACGACTCACTATAGGG 3'测序引物: 根据序列设计引物 质粒简介 The pRL-TK Vector may be used in combination with any experimental reporter vector to cotransfect mammalian cells. However, it is important to realize that trans effects between promoters on cotransfected plasmids can potentially affect reporter gene expression. This is primarily of concern when either the control or experimental reporter vector, or both, contain very strong promoter/enhancer elements.The occurrence and magnitude of such effects will depend on several factors: i) the combination and activities of the genetic regulatory elements present on the cotransfected vectors, ii) the relative ratio of experimental vector to control vector introduced into the cells, and iii) the type of cell transfected.

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