p406ADH1 BioVector NTCC质粒载体菌种细胞基因保藏中心

p406ADH1 BioVector NTCC质粒载体菌种细胞基因保藏中心
Vector backbone pRS406
(Search Vector Database)
Backbone manufacturer Sikorski & Hieter, Genetics 122:19-27 (1989)
Backbone size w/o insert (bp) 4283
Vector type Bacterial Expression, Yeast Expression
Selectable markers URA3
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Copy number High Copy
GENE/INSERT

Gene/Insert name ADH1 promoter + polylinker + CYC1 terminator
Alt name alcohol dehydrogenase
Species S. cerevisiae (budding yeast)
Insert Size (bp) 1795
Mutation ADH1 promoter is between SacI and XbaI restriction sites.
Entrez Gene ADH1 (a.k.a. YOL086C, ADC1)
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ cloning site SacI (not destroyed)
3′ cloning site KpnI (not destroyed)
5′ sequencing primer M13 Reverse
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net