首页 » 自 杀重组质粒pKC1139,pJQ200SK,pUTminiTn5,pORI280,大肠杆菌宿主细胞JM101,103,105,107,109,110,TOP10,10F,HB101

自 杀重组质粒pKC1139,pJQ200SK,pUTminiTn5,pORI280,大肠杆菌宿主细胞JM101,103,105,107,109,110,TOP10,10F,HB101

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  • 货  号:1224540
  • 产  地:北京
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  • pUT-miniTn5 Cm


  • 图谱Map



The mini-Tn5 are mobile elements able to transpose to the chromosome from a delivery plasmid, by means of the transposase activity encoded by the tnp* gene, which is present in cis in the plasmid but external to the mini-Tn5 element. pUT mini-Tn5 vectors can be used to insert any DNA fragment in the bacterial chromosome, by cloning it within the mini-Tn5 region. Due to the loss of the tnp* gene after insertion, minitransposons are stably integrated into the chromosome and inherited. The different pUTmini-Tn5 vectors can then be used for repeated insertion events and therefore multiple insertions in the same strain are only limited by the availability of distinct selection markers.

Advantages:

  • Easy and stable insertion of heterologous DNA in the chromosome of the strain of interest, after few simple genetic manipulations.

  • Wide range of inserted fragment sizes.

  • Possibility of multiple insertions in the same strain.

  • Easy modification of the cloning vector.

  • Use of cleavage sites of the rare cutters NotI and SfiI.

  • Wide range of Gram negative recipient strains.


Some of the most common applications of pUTmini-Tn5 vectors are:

  • Cloning.

  • Insertional mutagenesis.

  • Promoter probing.

  • Generation of conditional phenotypes and non-polar mutations.

  • Analysis of transcriptional terminators.

  • Environmental applications: tagging with marker genes for monitoring of released microorganisms and/or transcriptional regulation in the environment.


  • ---------------------

  • pUT mini-Tn5 Cm kit

    • pUT mini-Tn5 Cm, 2 μg.

    • E. coli DH5a lpir, stab.

    • E. coli S17-1 lpir, stab.

    • E. coli DH5a(pRK2013), stab.

    • TSS solution, 1.5ml.


    pUT mini-Tn Km kit

    • pUT mini-Tn5 Km, 2 μg.

    • E. coli DH5a lpir, stab.

    • E. coli S17-1 lpir, stab.

    • E. coli DH5a(pRK2073), stab.

    • TSS solution, 1.5ml.


    pUT mini-Tn Sm/Sp kit

    • pUT mini-Tn5 Sm/Sp, 2 μg.

    • E. coli DH5a lpir, stab.

    • E. coli S17-1 lpir, stab.

    • E. coli DH5a(pRK2013), stab.

    • TSS solution, 1.5ml.


    pUT mini-Tn Tc kit

    • pUT mini-Tn5 Tc, 2 μg.

    • E. coli DH5a lpir, stab.

    • E. coli S17-1 lpir, stab.

    • E. coli DH5a(pRK2013), stab.

    • TSS solution, 1.5ml.


    PANC-1 mini-Tn5 package

    • pUT mini-Tn5 Km, 2 μg.

    • pUT mini-Tn5 Cm, 2 μg.

    • pUT mini-Tn5 Sm/Sp, 2 μg.

    • pUT mini-Tn5 Tc, 2 μg.


    PANC-1plus miniTn5 package

    • pUT mini-Tn5 Km, 2 μg.

    • pUT mini-Tn5 Cm, 2 μg.

    • pUT mini-Tn5 Sm/Sp, 2 μg.

    • pUT mini-Tn5 Tc, 2 μg.

    • E. coli DH5a lpir, stab.

    • E. coli S17-1 lpir, stab.

    • E. coli DH5a(pRK2013), stab.

    • E. coli DH5a(pRK2073), stab.

    • TSS solution, 1.5ml.


    pUT mini-Tn5Tel kit

    • pUT mini-Tn5Tel vector, 2 μg.

    • Potassium Tellurite, 100mg.

    • E. coli DH5a lpir, stab.

    • E. coli S17-1 lpir, stab.

    • E. coli DH5a(pRK2013), stab.

    • TSS solution, 1.5ml.



另有

Tn5转座子载体 pUT-miniTn5 Cm,pUT-miniTn5 Tel
Tn10 delivery vector pHV1248,
mini-Tn10转座子载体pIC333, pIJ702等;
mini-Tn7转座子载体pTNS3, pEVS107, pPS2737, pUC18-mini-Tn7-LAC, pUC18R6KT Mini-Tn7, mini-Tn7-Tel,



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电邮
Biovector@163.com
电话18901268599

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原核表达载体

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资源名称系统
pET-3a(+)大肠杆菌表达质粒
pET-3c-sumo大肠杆菌表达质粒
pET-3d(+)大肠杆菌表达质粒
pET-11a(+)大肠杆菌表达质粒
pET-12a(+)大肠杆菌表达质粒
pET-15b(+)大肠杆菌表达质粒
pET-16b(+)大肠杆菌表达质粒
pET-17b(+)大肠杆菌表达质粒
pET-19b(+)大肠杆菌表达质粒
pET-20b(+)大肠杆菌表达质粒
pET-21a(+)大肠杆菌表达质粒
pET-21b(+)大肠杆菌表达质粒
pET-21d(+)大肠杆菌表达质粒
pET-22b(+)大肠杆菌表达质粒
pET-23a(+)大肠杆菌表达质粒
pET-23b(+)大肠杆菌表达质粒
pET-24a(+)大肠杆菌表达质粒
pET-25b(+)大肠杆菌表达质粒
pET-26b(+)大肠杆菌表达质粒
pET-27b(+)大肠杆菌表达质粒
pET-28a(+)大肠杆菌表达质粒
pET-28b(+)大肠杆菌表达质粒
pET-28c(+)大肠杆菌表达质粒
pET-28a(+)-sumo大肠杆菌表达质粒
pET-28a(+)-GFP大肠杆菌表达质粒
pET-28a(+)-SMT3大肠杆菌表达质粒



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