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pPMQAK1 BioVector NTCC质粒载体菌种细胞基因保藏中心
RSF1010 replicon from pAWG1.1 was PCR amplified with pAWG02-MunI-f and pAWG02-KpnI-r. A 2434bp fragment containing BioBrick cloning site and cassettes encoding resistance for ampicillin and kanamycin were PCR-amplified from pSB1AK3-BBa_B0015 with pSB03-KpnI-f and pSB03-MunI-r primers (see publication for primer sequences). These PCR products were cloned together to form the plasmid pPMQAK1-BBa_B0015. The BioBrick part BBa_B0015 was exchanged with part BBa_P1010 (encoding ccdB) using the EcoRI and PstI sites of the BioBrick cloning site.
pPMQAK1 multiple cloning site: AatII-EcoRI-XbaI-ccdB gene-BamHI-SpeI-PstI.
The ccdB gene is removed by cloning a BioBrick part into the cloning site.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net
RSF1010 replicon from pAWG1.1 was PCR amplified with pAWG02-MunI-f and pAWG02-KpnI-r. A 2434bp fragment containing BioBrick cloning site and cassettes encoding resistance for ampicillin and kanamycin were PCR-amplified from pSB1AK3-BBa_B0015 with pSB03-KpnI-f and pSB03-MunI-r primers (see publication for primer sequences). These PCR products were cloned together to form the plasmid pPMQAK1-BBa_B0015. The BioBrick part BBa_B0015 was exchanged with part BBa_P1010 (encoding ccdB) using the EcoRI and PstI sites of the BioBrick cloning site.
pPMQAK1 multiple cloning site: AatII-EcoRI-XbaI-ccdB gene-BamHI-SpeI-PstI.
The ccdB gene is removed by cloning a BioBrick part into the cloning site.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net
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