人正常阴道上皮细胞HNVEC/HL-016 BioVector NTCC质粒载体菌种细胞基因保藏中心

人正常阴道上皮细胞HNVEC/HL-016 BioVector NTCC质粒载体菌种细胞基因保藏中心
Human normal vaginal epithelial cells HNVEC/HL-016.
The primary isolated culture method comprises the following steps: removing fat in a para-carcinoma tissue sample excised from a vaginal carcinoma patient, digesting, adding dispase and DNase I to act, filtering, centrifugating to collect the cells, resuspending the cells by using an HL culture medium, inoculating, and culturing. The subculture method comprises the following steps: carrying out cell proliferation until the abundance is 70-90%, carrying out pancreatin-EDTA (ethylene diamine tetraacetic acid) digestion, and neutralizing with a DMEM (dulbecco's modified eagle medium); and centrifugating to collect the cells, resuspending the cells by using an HL culture medium, inoculating, and culturing. The cells can be used for physiological research of human normal cells, drug toxicity research and detection of in-vitro normal cells, and research of pathogenesis of vagina/cervix-related diseases (including vaginal carcinoma, cervical carcinoma and infectious diseases).

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