pBEN276革兰氏阴性菌Tn7转座子Luc荧光表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pBEN276革兰氏阴性菌Tn7转座子Luc荧光表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
pBEN276革兰氏阴性菌Tn7转座子Lux荧光表达载体，带有luxCDABE荧光报告基因，Tn转座子元件
Intergration of luciferase operon in Tn7 insertion site of Gram negative bacteria
Vector backbone pGRG25
(Search Vector Database)
Backbone manufacturer Nancy Craig - Johns Hopkins University (Addgene BioVector-016665)
Backbone size w/o insert (bp) 12500
Total vector size (bp) 19671
Vector type Luciferase
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 30°C
Growth Strain(s) DH5alpha
Copy number Low Copy
GENE/INSERT

Gene/Insert name luxCDABE
Species Photorhabdus luminescens
Insert Size (bp) 6200
GenBank IDAF403784
Promoter frr
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ cloning site XhoI (not destroyed)
3′ cloning site NotI (not destroyed)
5′ sequencing primer AATTGCCCGTCGTATTAAAG
3′ sequencing primer GTAGCGTCGTAAGCTAATAC
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net