HCT116 P53-/- cells基因敲除细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

HCT116 P53-/- cells基因敲除细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Cell Line
HCT116 P53 (-/-)
Functional Area
P53 signalling
Primary Reference
Bunz, F., et al. (1998). Requirement for p53 and p21 to sustain G2 arrest after DNA damage. Science 282(5393):1497-1501.
Related References
Matoba, S. et al. (2006). p53 regulates mitochondrial respiration. Science 312(5780):1650-3; Ravi, R. et al. (2000) Regulation of tumor angiogenesis by p53-induced degradation of hypoxia-inducible factor 1alpha. Genes Dev. 14(1):34-44.
General Information
Tissue
Colon
Disease
Colorectal carcinoma
Gender
Male
Key Endogenous Mutations
CDKN2A, CTNNB1, KRAS, PIK3CA, MLH1
STR Profile
Amelogenin: X,Y ; CSF1PO: 7,10 ; D13S317: 10,12 ; D16S539: 11,13 ; D5S818: 10,11 ; D7S820: 11,12 ; THO1: 8,9 ; TPOX: 8,9 ; vWA: 17,22; ATCC
Organism
Homo sapiens
Cell Line Characteristics
Gene ID
7157
Link To NCBI
http://www.ncbi.nlm.nih.gov/gene?term=7157
Genomic Locus
17p13.1
CVT Selection Condition
G418 resistant – 0.4mg/ml ; Hygromycin resistant – 0.1mg/ml
Quality Control
Genotype
P53 (-/-)
Cell Culture
Revival
Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 75cm2 flask containing pre-warmed media.
Media Type
RPMI 1640 including 2mM L-glutamine and 25mM sodium bicarbonate, supplemented with 10% FBS.
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:10-1:20.
Biosafety Level
1
Freeze Medium
Complete growth media 45%; USDA approved FBS 50%; DMSO 5%

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