Agrobacterium radiobacter strain K1026菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

Agrobacterium radiobacter strain K1026菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
Agrobacterium radiobacter strain K1026 is a microbial pesticide that can be used to treat
germinating seeds or roots and stems of certain stone fruit and nut trees and ornamentals to
protect them from crown gall disease. Products are intended for use in the greenhouse or in
nurseries. EPA has approved the use of this pesticide until fall 2001 while it waits for
additional data to more fully characterize the identity of the microbe. However, based on the
available information, the use of the pesticide is not expected to have any adverse effects on
human health or the environment.
Agrobacterium radiobacter strain K1026, is the result of a simple genetic alteration of a
naturally occurring bacterium Agrobacterium radiobacter strain K84. Except for the deletion
of a small portion of DNA removed in K84, the two strains are essentially identical and have
the same characteristics. Removal of this genetic material prevents the transfer of resistance
from strain K1026 to other strains. The naturally occurring bacterium Agrobacterium
radiobacter strain K84 is widespread in soil and found near plant roots. A toxic compound
produced by both K1026 and K84 controls certain other Agrobacterium spp. that cause
crown gall disease.
Based on review of the available toxicology and pathogenicity data and other information
related to Agrobacterium radiobacter strain K1026, EPA finds that this microbe is not likely
to produce adverse effects in humans. A very similar organism, Agrobacterium radiobacter
strain K84, has been used for approximately twenty years without any reports of adverse
effects or reactions. Agrobacterium radiobacter strains K1026 and K84 are not known to
cause any infectious diseases in humans. This microbial pesticide is not intended for use on
food crops. Consequently, it is not likely to pose a hazard to human adults, infants, children,
or other sensitive subpopulations via the dietary route of exposure. The potential aggregate
exposure, derived from non-dietary and non-occupational exposure, should be minimal or
non-existent. EPA is requiring growth temperature variation measurements to more
definitively characterize risks to human health.
The Tier 1 ecotoxicology studies were waived based on the derivation of Agrobacterium
radiobacter strain K1026 from strain K84, minimal exposure to non-target organisms, and no
evidence of any unintended effects on non-target species by strain K84. Based upon this
evaluation, the EPA has determined that Agrobacterium radiobacter strain K1026 is likely to
pose only a minimal risk to the environment or non-target organisms. EPA is requiring substrate utilization tests with K1026 and K84 to alleviate discrepancies in previously
submitted tests and confirm the lineage of K1026.


Medium Nutrient agar or nutrient broth
Growth Conditions
Temperature: 30.0°C
Atmosphere: Aerobic
Propagation Procedure
1. Open vial according to enclosed instructions.
2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or
1.0 mL pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 24 hours.

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net