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p482.6 plasmid质粒 BioVector NTCC质粒载体菌种细胞基因保藏中心
Designations p482.6
GenBank Number
X01179
Species Homo sapiens, human
Applications
The 6.2 and 4.8 kb fragments are detected by hybridizing a 1.1 kb EcoRI/SstI fragment to genomic DNA digested with XbaI and KpnI. KpnI is used to distinguish a non-intron 22-sequence migrating at 6.6 kb.
Vector
Construct size (kb): 12.19999980926514
Insert
DNA: genomic
Insert lengths(kb): 9.600000381469727
Gene product: coagulation factor VIIIc, procoagulant component (hemophilia A) [F8C]
Alleles: F3, E1, E1, E2, F1, F2, A1, A2
Insert Size (kb) 9.600
Biosafety Level 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 2.6; HindIII--9.6, 2.6; PstI--3.5, 2.6, 2.3, 1.2, 0.8, 0.5, 0.3; BamHI--7.3, 4.6; AccI--5.2, 2.9, 1.4, 1.4, 0.45.
The insert is an EcoRI fragment of a portion of intron 22. The polymorphic XbaI site is included in the sequence recognized by the probe.
The non-intron 2 band (6.6 kb in a KpnI/XbaI double digest) appears to be more precisely 2 bands at 6.6 and 6.8 kb which are X-chromosomal in origin and may be polymorphic (5.4 + 1.4 and 5.2 + 1.4).
The 6.2 and 1.4 kb fragments are detected by hybridizing a 1.6 kb BstXI fragment to genomic DNA digested with XbaI and KpnI. This fragment seems superior in detecting the XbaI polymorphism.
The 6.2 and 4.8 kb fragments are detected by hybridizing a 1.1 kb EcoRI/SstI fragment to genomic DNA digested with XbaI and KpnI. KpnI is used to distinguish a non-intron 22-sequence migrating at 6.6 kb.
References
Lillicrap DP, et al. Variation of the non-factor VIII sequences detected by a probe from intron 22 of the factor VIII gene. Blood 75: 139-143, 1990. PubMed: 1967212
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net
Designations p482.6
GenBank Number
X01179
Species Homo sapiens, human
Applications
The 6.2 and 4.8 kb fragments are detected by hybridizing a 1.1 kb EcoRI/SstI fragment to genomic DNA digested with XbaI and KpnI. KpnI is used to distinguish a non-intron 22-sequence migrating at 6.6 kb.
Vector
Construct size (kb): 12.19999980926514
Insert
DNA: genomic
Insert lengths(kb): 9.600000381469727
Gene product: coagulation factor VIIIc, procoagulant component (hemophilia A) [F8C]
Alleles: F3, E1, E1, E2, F1, F2, A1, A2
Insert Size (kb) 9.600
Biosafety Level 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--9.6, 2.6; HindIII--9.6, 2.6; PstI--3.5, 2.6, 2.3, 1.2, 0.8, 0.5, 0.3; BamHI--7.3, 4.6; AccI--5.2, 2.9, 1.4, 1.4, 0.45.
The insert is an EcoRI fragment of a portion of intron 22. The polymorphic XbaI site is included in the sequence recognized by the probe.
The non-intron 2 band (6.6 kb in a KpnI/XbaI double digest) appears to be more precisely 2 bands at 6.6 and 6.8 kb which are X-chromosomal in origin and may be polymorphic (5.4 + 1.4 and 5.2 + 1.4).
The 6.2 and 1.4 kb fragments are detected by hybridizing a 1.6 kb BstXI fragment to genomic DNA digested with XbaI and KpnI. This fragment seems superior in detecting the XbaI polymorphism.
The 6.2 and 4.8 kb fragments are detected by hybridizing a 1.1 kb EcoRI/SstI fragment to genomic DNA digested with XbaI and KpnI. KpnI is used to distinguish a non-intron 22-sequence migrating at 6.6 kb.
References
Lillicrap DP, et al. Variation of the non-factor VIII sequences detected by a probe from intron 22 of the factor VIII gene. Blood 75: 139-143, 1990. PubMed: 1967212
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net
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