pCMV-TagBFP BioVector NTCC质粒载体菌种细胞基因保藏中心

pCMV-TagBFP BioVector NTCC质粒载体菌种细胞基因保藏中心
Location of features
PCMV IE: 1-589
Enhancer region: 59-465
TATA box: 554-560
Transcription start point: 583
TagBFP
Kozak consensus translation initiation site: 600-610
Start codon (ATG): 607-609; Stop codon: 1384-1386
Last amino acid in TagBFP: 1303-1305
MCS: 1306-1383
SV40 early mRNA polyadenylation signal
Polyadenylation signals: 1526-1531 & 1555-1560
mRNA 3’ ends: 1564 & 1576
f1 single-strand DNA origin: 1623-2078
Bacterial promoter for expression of Kanr gene
-35 region: 2140-2145; -10 region: 2163-2168
Transcription start point: 2175
SV40 origin of replication: 2419-2554
SV40 early promoter
Enhancer (72-bp tandem repeats): 2252-2323 & 2324-
2395
21-bp repeats: 2399-2419, 2420-2440 & 2442-2462
Early promoter element: 2475-2481
Major transcription start points: 2471, 2509, 2515 &
2520
Kanamycin/neomycin resistance gene
Neomycin phosphotransferase coding sequences:
Start codon (ATG): 2603-2605; Stop codon: 3395-3397
G->A mutation to remove Pst I site: 2785
C->A (Arg to Ser) mutation to remove BssH II site: 3131
Herpes simplex virus (HSV) thymidine kinase (TK)
polyadenylation signal
Polyadenylation signals: 3633-3638 & 3646-3651
pUC plasmid replication origin: 3982-4625

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net