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pBABe-HA-ER-IPpoI BioVector NTCC质粒载体菌种细胞基因保藏中心
Vector backbone pBABE-Puro
(Search Vector Database)
Backbone manufacturer margenstern and L (1991) PMID Z194165
Backbone size w/o insert (bp) 5169
Vector type Mammalian Expression, Retroviral
Selectable markers Puromycin
GROWTH IN BACTERIA
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) Stbl3
Copy number Unknown
GENE/INSERT
Gene/Insert name I-PpoI
Species Physarum polycephalum
Insert Size (bp) 1514
Mutation none
GenBank IDPMD 17473858
Tags / Fusion Proteins
HA (N terminal on insert)
ER (N terminal on insert)
CLONING INFORMATION
Cloning method Restriction Enzyme
5′ cloning site BamHI (destroyed during cloning)
3′ cloning site BamHI (destroyed during cloning)
5′ sequencing primer PBABE 5'
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net
Vector backbone pBABE-Puro
(Search Vector Database)
Backbone manufacturer margenstern and L (1991) PMID Z194165
Backbone size w/o insert (bp) 5169
Vector type Mammalian Expression, Retroviral
Selectable markers Puromycin
GROWTH IN BACTERIA
Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) Stbl3
Copy number Unknown
GENE/INSERT
Gene/Insert name I-PpoI
Species Physarum polycephalum
Insert Size (bp) 1514
Mutation none
GenBank IDPMD 17473858
Tags / Fusion Proteins
HA (N terminal on insert)
ER (N terminal on insert)
CLONING INFORMATION
Cloning method Restriction Enzyme
5′ cloning site BamHI (destroyed during cloning)
3′ cloning site BamHI (destroyed during cloning)
5′ sequencing primer PBABE 5'
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net
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