pIC333革兰氏阳性菌-枯草芽孢杆菌Tn10转座子载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pIC333革兰氏阳性菌-枯草芽孢杆菌Tn10转座子载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
pIC333革兰氏阳性菌-枯草芽孢杆菌Tn10转座子载体，带有Mini-Tn10转座子，Spc壮观霉素抗性和Em红霉素抗性，温敏性复制子在革兰氏阳性菌宿主中高于35度失活，经实验证实是枯草芽孢杆菌的有效mini-Tn10转座子载体。
Description
An improved mini-Tn10 delivery plasmid pIC333, 7 kb, contains (i) a 2.4-kb mini-Tn10 containing a ColEl origin and an SPC resistance gene, (ii) an ERY resistance gene, (iii) a thermosensitive origin of replication for gram-positive hosts (inactive at temperatures higher than 35°C) , and (iv) a Tn10 transposase gene with mutations previously shown to decrease the target specificity of transposition in E. coli. Experiments showed that pIC333 is an efficient mini-Tn10 delivery vector in B. subtilis.

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