pADH1-HA-LEU2酿酒酵母组成型表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pADH1-HA-LEU2酿酒酵母组成型表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心
The pADH1-HA-LEU2 vector expresses proteins at high levels from the constitutive ADH1
promoter (PADH1); transcription is terminated at the ADH1 transcription termination signal (TADH1).
pADH1-HA-LEU2 also contains the T7 promoter, an HA epitope tag, and a MCS. pADH1-HA-LEU2 replicates autonomously in both E. coli and S. cerevisiae from the pUC and 2 ori, respectively. The vector carries Ampr for selection in E. coli and the LEU2 nutritional marker for selection in yeast.
The T7 promoter is used for in vitro transcription and translation of the epitope tagged fusion protein and also provides a binding site for sequencing using the T7 Sequencing Primer.

Location of features:
• Full-length S. cerevisiae ADH1 promoter (PADH1): 7–1479
• GAL4 AD polypeptide with SV40 Nuclear Localization Signal (NLS)
NLS: 1501–1557
GAL4 amino acids 768–881: 1561–1899
• T7 RNA polymerase promoter: 1905–1927
• HA epitope tag: 1942–1968
• Multiple Cloning Sites: 1969–2041
• Transcription termination signal
Fragment carrying the S. cerevisiae ADH1 terminator (TADH1): 2280–2605
• LEU2 coding sequences: 3814–2723
• pUC plasmid replication origin: 4581–5418
• Ampicillin resistance gene: 6432–5575
• Yeast 2 replication origin: 6998–7988
Location of primers:
• T7 Sequencing Primer: 1905–1925
Propagation in E. coli:
• Suitable host strains: DH5, DH10 & other general purpose strains
• Selectable marker: plasmid confers resistance to ampicillin (100 g/ml) to E. coli hosts
• E. coli replication origin: pUC
• Copy number: ~500
• Plasmid incompatibility group: pMB1/Col E1
Propagation in S. cerevisiae:
• Suitable host strains: Y187(), Y190(a), SFY526(a), CG1945(a), HF7c(a), or AH109(a)
• Selectable marker: LEU2
• S. cerevisiae origin: 2 

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net