pLDR11 BioVector NTCC质粒载体菌种细胞基因保藏中心

pLDR11 BioVector NTCC质粒载体菌种细胞基因保藏中心
This is a set of Escherichia coli integrating vectors that differ in their selectable markers (kanamycin,
chloramphenicol, tetracycline). Also included is a strain containing a compatible helper plasmid (pLDR8)
that provides the lambda Int necessary for attP/attB integration.
pLDR11 plasmid in Escherichia coli
Description: This vector contains the attP sequence for integrating DNA into the lambda attachment site
attB. It requires the lambda inegrase (int) such as that provided by pLDR8. When a recombinant construct
is digested with NotI, the origin is separated from the insert. Each fragment has a distinct selectable marker.
After religation to form originfree
circles, the products are tranformed into bacteria with pLDR8. Expression
of int in pLDR8 is regulated by lambda PR, by the temperaturesensitive
cI857 repressior. Replication of
pLDR8 is also temperature sensitive. Therefore, at 30°C, replication of the plasmid is normal and int is not
expressed. At 42°C, int is expressed but pLDR8 is no longer replicated, resulting in loss of int after a few
cell generations. The order of the major features in this plasmid are: NotI ampR
ClaI/
MCS/EcoRI attP
NotI
pMB1
ori tetR.
Plasmid
28: 1424,
1992
Vector information:
Vector size (kb): 4.1
Markers: ampR, tetR
Replicon: pMB1
Cloning sites: ClaI HindIII XbaI SmaI SacI EcoRI
Construction: pT77,
λ attP, pBR322 tet
Distribution host: Escherichia coli WM1202
Recommended growth media: LB + ampicillin (50 μg/mL)
Temperature: 37°C
Notes: Restriction digests of the vector gave the following sizes (in kb): EcoRI/Bg1II 3.6,
0.5; BamHI 2.2,
1.9; XbaI 4.1;
NotI 2.3,
1.8.
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net