pLDR8 BioVector NTCC质粒载体菌种细胞基因保藏中心

pLDR8 BioVector NTCC质粒载体菌种细胞基因保藏中心
This is a set of Escherichia coli integrating vectors that differ in their selectable markers (kanamycin,
chloramphenicol, tetracycline). Also included is a strain containing a compatible helper plasmid (pLDR8)
that provides the lambda Int necessary for attP/attB integration.

Description: pLDR8 is compatible with, and provides int function for, the vectors pLDR9, pLDR10, and
pLDR11. Expression of int in pLDR8 is regulated by lambda PR, by the temperature sensitive cI857
repressor. Replication of pLDR8 is also temperature sensitive. Therefore, at 30°C, replication of the plasmid
is normal and int is not expressed. At 42°C, int is expressed but pLDR8 is no longer replicated, resulting in
loss of int after a few cell generations. The order of the major features in this plasmid are: EcoRI pSC101
ori neoR
PstI
cI857
PR
prometer int.
Plasmid
28: 1424,
1992
Note: Temperatureresistant
derivatives arise frequently. It is important to isolate a clone that is
temperaturesensitive
and kanamycinresistant
before use.
Vector information:
Vector size (kb): 7.6
Markers: kanR
Distribution host: Escherichia coli DH5α
Recommended growth media: LB + kanamycin (25 μg/mL)
Temperature: 30°C (see notes above)
Notes: Restriction digests of the vector gave the following sizes (in kb): EcoRI/PstI 5.4,
2.2; BamHI 4.2,
3.4; PstI 7.6;
HindIII 5.7,
1.9
ATCC
Staff
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net