Yarrowia lipolytica PO1f BioVector NTCC质粒载体菌种细胞基因保藏中心

Yarrowia lipolytica PO1f BioVector NTCC质粒载体菌种细胞基因保藏中心
Biovector690322 Yarrowia lipolytica PO1f 1Vial, Storage:4℃

Strain Designations PO1f
Synonym Endomycopsis lipolytica Wickerham et al., Saccharomycopsis lipolytica (Wickerham et al.) Yarrow
Alternate State Candida lipolytica (Harrison) Diddens et Lodder
Application transformation host
Medium YPD Medium
Broth Medium
YPD Broth(BD 242820)……………………....50 g
DI Water………………………………………..1000 ml
Or for solid medium:
Agar Medium
YPD Agar(BD 242720)………………………..65 g
DI Water………………………………………...1000 ml
Dispense into appropriate vessel and autoclave at 121ºC.
*YPD is commercially available from BD. To make medium from scratch, follow
formulation below:
Yeast Extract……………………………………10.0 g
Peptone…………………………………………20.0 g
Dextrose………………………………………...20.0 g
Agar (if needed)………………………………..20.0 g
DI Water………………………………………...1000 ml
Adjust for final pH of 5.6. Autoclave at 121oC.
Growth Conditions Temperature: 24°C to 26°C
Atmosphere: Typical aerobic
Genotype MATA ura3-302 leu2-270 xpr2-322 axp2-deltaNU49 XPR2::SUC2
Phenotype Leu-, Suc-, Ura-
Comments Unable to produce extracellular protease
Morphology On YEPD medium at 25°C after 5 days, colonies creamy white to buff, smooth becoming tomentose, margin entire becoming filamentous. Cells hyaline, subglobose to ovoid, smooth, 5.5-10.5 X 3-6 µm. Pseudohyphae present.


Sequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence
TATTACAGCGTCATTTTATCTCAATTATAACTATCAACAACGGATCTCTTGGCTCTCACATCGATGAAGAACGCAGCGAACCGCGATATTTTTTGTGACTTGCAGATGTGAATCATCAATCTTTGAACGCACATTGCGCGGTATGGCATTCCGTACCGCACGGATGGAGGAGCGTGTTCCCTCTGGGATCGCATTGCTTTCTTGAAATGGATTTTTTAAACTCTCAATTATTACGTCATTTCACCTCCTTCATCC


D1D2 region of the 28S ribosomal RNA gene
AGAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAACCCTCGGGATTGTAATTTGAAGATTTGGCATTGGAGAAAGCTAACCCAAGTTGCTTGGAATAGTACGTCATAGAGGGTGACAACCCCGTCTGGCTAACCGTTCTCCATGTATTGCCTTATCAAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAAGTGGGTGGTAAACTCCATCTAAAGCTAAATACTGGTGAGAGACCGATAGCGAACAAGTACTGTGAAGGAAAGGTGAAAAGAACTTTGAAAAGAGAGTGAAATAGTATGTGAAATTGTTGATAGGGAAGGAAATGAGTGGAGAGTGGCCGAGGTTTCAGCCGCCCCTCGTGGGCGGTGTACTGCCGACGCCGAGTCATCGATAGCGAGACGAGGGTTACAAATGGGAGCGCCTT

Morphology
On YEPD medium at 25°C after 5 days, colonies creamy white to buff, smooth becoming tomentose, margin entire becoming filamentous. Cells hyaline, subglobose to ovoid, smooth, 5.5-10.5 X 3-6 µm. Pseudohyphae present.

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