VYH10 strain酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

VYH10 strain酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
YVH10, Saccharomyces cerevisiae
Cat No.: BioVector690322

Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain Designations YVH10
Alternate State Candida robusta Diddens et Lodder
Application Used in the yeast display method to isolate novel proteins based on various biological functions
Genotype MATalpha PDI1::GAPDH-PDI1::LEU2 ura3- 52 trp1 leu2-delta200 his3-delta200 pep4::HIS3 prbd1.6R can1 GAL
Comments YVH10 (Ura-, Trp-) is BJ5464 and is MAT-alpha. The other markers are: ura3-52 (a Ty element insertion with no detectable background reversion frequency), trp1 (an amber point mutation), leu2-delta200, his3-delta200, pep4:HIS3, prbd1.6R, can1, GAL. YVH10 has the yeast PDI gene integrated in tandem with the endogenous copy and is under the constitutive glyceraldehydes (GADPH) promoter with Leu2 as a selectable marker.
Morphology On YM medium at 30°C for 2 days, colonies creamy white to dingy white, butyrous, smooth, slightly raised, margin entire. Cells globose to subglobose, hyaline, smooth, contain oil droplets, 4-6 X 3-5 µm. Pseudohyphae not observed.


Isolation From parent strain BJ5464


Medium Emmons' modification of Sabouraud's agar
YM agar or YM broth
YEPD
Growth Conditions Temperature: 30°C
Atmosphere: Typical aerobic

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