BY4741 strain酿酒酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心

BY4741 strain酿酒酵母菌株 BioVector NTCC质粒载体菌种细胞基因保藏中心
BY4741 strain酿酒酵母菌株

Genotype
MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0

Description
S288C-derivative laboratory strain
BY4741 is part of a set of deletion strains derived from S288C in which commonly used selectable marker genes were deleted by design in order to minimize or eliminate homology to the corresponding marker genes in commonly used vectors without significantly affecting adjacent gene expression. The yeast strains were all directly descended from FY2, which is itself a direct descendant of S288C. Variation between BY4741 and S288C is miniscule. BY4741 was used as a parent strain for the international systematic Saccharomyces cerevisiae gene disruption project.
• Brachmann CB, et al. (1998) Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14(2):115-32 PMID:9483801
• Winston F, et al. (1995) Construction of a set of convenient Saccharomyces cerevisiae strains that are isogenic to S288C. Yeast 11(1):53-5 PMID:7762301


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