首页 » pIEFBPR枯草芽孢杆菌整合型表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pIEFBPR枯草芽孢杆菌整合型表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

  • 价  格:¥38935
  • 货  号:pIEFBPR枯草芽孢杆菌整合型表达载体
  • 产  地:北京
点击询问我要采购
 竭诚为您服务!
BioVector NTCC典型培养物保藏中心
联系人:Dr.Xu, Biovector NTCC Inc.

电话:400-800-2947 工作QQ:1843439339 (微信同号)

邮件:Biovector@163.com

手机:18901268599

地址:北京

已注册
 

pIEFBPR枯草芽孢杆菌整合型表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心


A novel method for the directed genetic manipulation of the Bacillus subtilis chromosome free of any selection marker. Our new approach employed the Escherichia coli toxin gene mazF as a counter-selectable marker. The mazF gene was placed under the control of an isopropyl-?-D-thiogalactopyranoside (IPTG)-inducible expression system and associated with a spectomycin-resistance gene to form the MazF cassette, which was flanked by two directly-repeated (DR) sequences. A double-crossover event between the linearized delivery vector and the chromosome integrated the MazF cassette into a target locus and yielded an IPTG-sensitive strain with spectomycin-resistance, in which the wild-type chromosome copy had been replaced by the modified copy at the targeted locus. Another single-crossover event between the two DR sequences led to the excision of the MazF cassette and generated a strain with IPTG resistance, thereby realizing the desired alteration to the chromosome without introducing any unwanted selection markers. We used this method repeatedly and successfully to inactivate a specific gene, to introduce a gene of interest and to realize the in-frame deletion of a target gene in the same strain. As there is no prerequisite strain for this method, it will be a powerful and universal tool.


BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话:+86-010-53513060
网址:www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net

您正在向 biovector.net  发送关于产品 pIEFBPR枯草芽孢杆菌整合型表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心 的询问

点击“立即发送”后,我们将在1个工作日内与您取得联系。