BNL.CL.2 cells小鼠胚胎肝细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

BNL.CL.2 cells小鼠胚胎肝细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心
BNL CL.2 cell line 小鼠胚胎肝细胞
Cat No.: NTCC690289

Organism Mus musculus, mouse
Tissue liver
Product Format frozen
Morphology epithelial
Culture Properties adherent
Disease normal
Age embryo
Strain BALB/c
Tumorigenic No, the cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Comments The line was selected to grow in medium containing ornithine and phenylalanine in place of arginine and tyrosine.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is Dulbecco's Modified Eagle's Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Subcultivation Ratio: 1:2 to 1:6
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
Cryopreservation Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions Temperature: 37°C
Atmosphere: 10% CO2
Deposited As Mus musculus
References Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934

BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net
【Supplier来源】BioVector NTCC Inc.
【Website网址】 http://www.biovector.net