LV-Cre BioVector NTCC质粒载体菌种细胞基因保藏中心

LV-Cre BioVector NTCC质粒载体菌种细胞基因保藏中心 The nlsCre was first cloned into the EcoRV site of a pBluescript II KS vector, and then inserted into the XbaI-SalI sites of a pRRL-derived lentiviral vector that contains a central polypurine tract (ppt) and the posttranscriptional element of the woodchuck hepatitis virus.
Vector backbone p156RRL
(Search Vector Database)
Backbone manufacturer Cell Genesys
Backbone size w/o insert (bp) 7424
Vector type Mammalian Expression, Lentiviral, Cre/Lox
Selectable markers none
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) Stbl3
Copy number Low Copy
GENE/INSERT

Gene/Insert name nlsCre
Alt name nls Cre
Alt name LV-Cre
Alt name cre
Species Enterobacteriophage P1
Insert Size (bp) 1136
Entrez Gene cre (a.k.a. P1_gp003)
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ cloning site XbaI (unknown if destroyed)
3′ cloning site SalI (not destroyed)
5′ sequencing primer LNCX
BioVector NTCC质粒载体菌种细胞蛋白抗体基因保藏中心
电话：+86-010-53513060
网址：www.biovector.net [Supplier来源] http://www.biovector.net