Human T-cell lymphoma Cells人T细胞淋巴瘤细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心

Human T-cell lymphoma Cells人T细胞淋巴瘤细胞 BioVector NTCC质粒载体菌种细胞基因保藏中心 Human T-cell lymphoma Cells
Organism Homo sapiens, human
Cell Type cutaneous T lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Disease Sezary Syndrome
Age 53 years adult
Gender male
Ethnicity Caucasian
Applications This cell line is a suitable transfection host.
Images
Clinical Data 53 years adult
Caucasian
male
Antigen Expression Antigen expression: CD4; Homo sapiens Ref
Receptor Expression Receptor expression: interleukin 2 (IL-2), expressed Ref
Genes Expressed interleukin 2, tumor necrosis factor alpha (TNF alpha), CD4; Homo sapiens
Tumorigenic Yes
Effects Yes, in nude mice when injected intracranially
Complete Growth Medium The base medium for this cell line is Iscove's Modified Dulbecco's Medium . To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 X 105 viable cells/ml. Maintain cultures at cell concentrations between 5 X 104 and 8 X 105 viable cells/mL. Maintain cell density at less than 1 X 106 cells/mL.
Medium Renewal: Two to three times weekly
Cryopreservation Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11,12
D5S818: 11,12
D7S820: 8,11
THO1: 8,9
TPOX: 8,9
vWA: 14,15
Population Doubling Time about 65 hours

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