NCTC 1469 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

NCTC 1469 cell line细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 NCTC 1469 cell line细胞株
Description: Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).

Species: Mouse

Tissue: Liver

Growth Properties: Adherent

Morphology: Epithelial

Growth Medium: NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)

Subculturing Procedure: Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10% CO2; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearance similar to bacterial contamination. Suspension cells are viable and can be used for further subculture.

Release Conditions: No

Depositor:

Originator: No

References: J Nat Cancer Inst 1952;12:1245; J Nat Cancer Inst 1957;18:701; J Nat Cancer Inst 1959;23:823

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