OVCAR-3 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

OVCAR-3 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 NIH:OVCAR-3 [OVCAR3] Cell Line
Cat No.: NTCC594777

Organism Homo sapiens, human
Tissue ovary
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 60 years
Gender female
Ethnicity Caucasian
Applications This cell line is a suitable transfection host.

NIH:OVCAR-3 is an appropriate model system in which to study drug resistance in ovarian cancer, and the presence of hormone receptors should be useful for the evaluation of hormonal therapy.
Karyotype The cell line is aneuploid human female, with chromosome counts in the sub to near-triploid range. Several normal chromosomes (N11, N13, N14, N15, N16, N17, and N22) are clearly under-represented. Many of these missing chromosomes are represented in the large number of cytogenetically altered chromosomes identified as marker chromosomes. In addition to the marker chromosomes, there are a large number of other structurally abnormal and unassignable chromosomes that are not recognized as markers. Random loss and gain of chromosomes from cell to cell are noted in the exact chromosome counts and in the analysis of the karyotypes.
Images
Derivation The NIH:OVCAR-3 line was established in 1982 by T.C. Hamilton, et al. from the malignant ascites of a patient with progressive adenocarcinoma of the ovary.
Clinical Data Caucasian
female
60 years
Receptor Expression Androgen receptor, positive; estrogen receptor, positive; progesterone receptor, positive
Tumorigenic Yes
Effects Yes, Forms colonies in soft agar
Yes, in nude mice inoculated subcutaneously with 10(7) cells
(Tumors developed within 21 days at 100% frequency (5/5).)
Comments Forms colonies in soft agar and has an abnormal karyotype.
Resistant to clinically relevant concentrations of adriamycin, melphalan and cisplatin.
Both cultured cells and xenografts exhibit androgen and estrogen receptors.
Xenograft models have been used to show that treatment with 17 beta estradiol can induce progesterone receptors in this human ovarian carcinoma.
Subculturing Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 2.0 to 3.0 mL of complete growth medium and aspirate cells by gently pipetting
5. Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 12
D5S818: 11,12
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 17
Isoenzymes AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
PGM1, 1
PGM3, 1
References Hamilton TC, et al. Characterization of a human ovarian carcinoma cell line (NIH:OVCAR-3) with androgen and estrogen receptors. Cancer Res. 43: 5379-5389, 1983. PubMed: 6604576
Rogan AM, et al. Reversal of adriamycin resistance by verapamil in human ovarian cancer. Science 224: 994-996, 1984. PubMed:6372095

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