pRMC2金黄色葡萄球菌四环素诱导表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心

pRMC2金黄色葡萄球菌四环素诱导表达载体 BioVector NTCC质粒载体菌种细胞基因保藏中心 Tetracycline-inducible expression vector for Staphylococcus aureus
Note--MCS is inverted in the article describing this plasmid.
sequencing results with the pRMC2 SEQF primer find the MCS is duplicated, presumably due to the cloning strategy.
Vector backbone pALC2073
(Search Vector Database)
Vector type Bacterial Expression ; tet inducible E. coli/Staphylococcus shuttle vector
GROWTH IN BACTERIA

Bacterial Resistance(s) Ampicillin
Growth Temperature 37°C
Growth Strain(s) DH5alpha
Growth instructions Chloramphenicol should be used when transformed in S. Aureus.
Copy number Unknown
CLONING INFORMATION

Cloning method Restriction Enzyme
5′ sequencing primer pRMC2 SEQF: ATTCAGGCTGCGCAAC
3′ sequencing primer pRMC2 SEQR: TTGTTGACATTATATCATTG
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