T84 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心

T84 cells细胞株 BioVector NTCC质粒载体菌种细胞基因保藏中心 T84 cell line
Cat No.: NTCC690089

Organism Homo sapiens, human
Tissue colon; derived from metastatic site: lung
Product Format frozen
Morphology epithelial
Culture Properties adherent
Disease colorectal carcinoma
Age 72 years
Gender male
Applications The T84 cell line is a transplantable human carcinoma cell line. It is also a suitable transfection host.

Karyotype The stemline modal chromosome number is 56, occurring at 28% with polyploidy at 12.4%. Eighteen markers are common to most metaphases examined. Normal X and chromosome 13 were absent; chromosomes 2, 4 and 22 were single-copied, and chromosome 12 was 4-copied. No Y chromosome was detected by Q band observation. DM occurred in nearly 50% of the cells.
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Derivation The T84 cell line is a transplantable human carcinoma cell line derived from a lung metastasis of a colon carcinoma in a 72-year-old male. Tumor tissue was inoculated subcutaneously and serially transplanted in BALB/c nude mice.
Clinical Data male

Receptor Expression neurotransmitter, expressed
peptide hormone, expressed
Genes Expressed carcinoembryonic antigen (CEA), 600 ng/mL per 106 cells per 10 days; keratin
Cellular Products carcinoembryonic antigen (CEA), 600 ng/ml per 10 exp6 cells per 10 days; keratin
Tumorigenic Yes
Effects Yes, in nude mice
(Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
Comments The original histological characteristics of the colon carcinoma were maintained throughout transplantation in nude mice.
After 23 passages in athymic mice, the T84 cell line was established. These cells grow to confluence as monolayers and exhibit tight junctions and desmosomes between adjacent cells.
They have receptors for many peptide hormones and neurotransmitters and maintain vectorial electrolyte transport.
This line exhibits tight junctions, and desmosomes between adjacent cells.
The cells are positive for keratin by immunoperoxidase staining.
Complete Growth Medium A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium with 2.5 mM L-glutamine, 95%; fetal bovine serum, 5%
Subculturing Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Cryopreservation Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
Growth Conditions: The cells should be maintained at high density (at least 1/4 confluency).
STR Profile Amelogenin: X
CSF1PO: 10
D13S317: 9
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 6,9
TPOX: 8,11
vWA: 17,18
Isoenzymes AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1-2
PGM1, 1
PGM3, 1

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